FastQCFastQC Report
Thu 13 Dec 2018
Test02_L001_R2_001.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameTest02_L001_R2_001.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800
Sequences flagged as poor quality0
Sequence length57-251
%GC39

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTTTCGTTCTTGATCAATGAAAACATCCTTGGCAAATGCTTTCGCAGTA39148.875No Hit
ACTTTCGTTCTTGATCAATGAAAACATCCTTGGCAAATGCTTTCGCAGTC496.125No Hit
ACTTTCGTTCTTGATTAATGAAAACATCCTGGGCAAATGCTTTCGCAGTA486.0No Hit
ACTTTCGTTCTTGATTAATGAAAACACTCTTGGAAAATGCTTTCGCAGTA405.0No Hit
ACTTTCGTTCTTGATTAATGAAAACATTCTTGGCAAATGCTTTCGCAGTA212.625No Hit
ACTTTCGTTCTTGATCAATGAAAACATCCATGGCAGATGCTTTCGCAGTA202.5No Hit
ACTTTCGTTCTTGATCAATGAGAACATCCTGGGTAAATGCCTTCGCAATT141.7500000000000002No Hit
ACTTTCGTTCTTGATCAGTGAAAACATCCCTGGCAAATGCTTTCGCAGTG121.5No Hit
ACTTTCGTTCTTGATTAATGAAAACATCCTTGGTAAATGCTTTCGCAGTA111.375No Hit
ACTTTCGTTCTTGATCAATGAAAACATCCTTGGTAAATGCTTTCGCAGTA101.25No Hit
ACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCATAA91.125No Hit
ACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGTA91.125No Hit
ACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCGCAGAA81.0No Hit
ACTTTCGTTCTTGATCAATGAGAACATCTTTGGCAAATGCTTTCGCAGTA81.0No Hit
ACTTTCGTTCTTGATCAATGAAAGCATCCTTGGCAAATGCTTTCGCAGTC70.8750000000000001No Hit
ACTTTCGTTCTTGATCAACAAAAACATCCTGGGTAGATGCCTTCGCAATT60.75No Hit
ACTTTCGTTCTTGATTAAAGAAAACATTCTTGGCAAATGCTTTCGCAGTT50.625No Hit
ACTTTCGTTCTTGATTAATGAAAACACTCTTGGCAAATGCTTTCGCAGTA50.625No Hit
ACTTTCGTTCTTGATTAATGAAAACATCCTTGACAAATGCTTTCGCAGAA50.625No Hit
ACTTTCGTTCTTGATCAATGAAAACGTTCTCGTGAAACGCCTTCGCATCC40.5No Hit
ACTTTCGTTCTTGATCAATGAAAACATCTTTGGCAAATGCTTTCGCAGTA40.5No Hit
ACTTTCGTTCTTGATCAATGAGAACATCTTTGGCAGATGCTTTCGCAGTA40.5No Hit
ACTTTCGTTCTTGATTAATAAAAACATCCTGGGCAGATGCTTTCGCAGTA40.5No Hit
ACTTTCGTTCTTGATCAATGAAAACATATTTGGCAAATGCTTTCGCAGTA30.375No Hit
ACTTTCGTTCTTGATCAGTGAAAACATCCCTGGCAAATGCTTTCGCAGTA30.375No Hit
ACTTTCGTTCTTGATTAATGAAAACACTCTTGGCAAGTGCTTTCGCAGTT30.375No Hit
ACTTTCGTTCTTGATTAATGAAAACATCCTTGGTAAATGCTTTCGCATGA30.375No Hit
ACTTTCGTTCTTGATCAATGCAAACATCCTTGGCAAATGCTTTCGCAGTA30.375No Hit
ACTTTCGTTCTTGATCAATGAAAACATTCTTGGCAAATGCTTTCGCAGTG30.375No Hit
ACTTTCGTTCTTGATCAATGAAAACATGATTGGCAAATGCTTTCGCAGCA30.375No Hit
ACTTTCGTTCTTGATTAAAGAAAACATTCTTGGCGAATGCGTTCGCAGTA30.375No Hit
ACTTTCGTTCTTGATTAATGAAAACATCCTTGATGAATGCTTTCGCAGTA30.375No Hit
ACTTTCGTTCTTGATCAATAAAAACATCCTGGGCAGATGCCTTCGCAATA30.375No Hit
ACTTTCGTTCTTGATTAATGAAAACATCTTTGGTAAATGCTTTCGCAGAA20.25No Hit
ACTTTCGTTCTTGATCAATGAAAACATCCTTGGCAAATGCTTTCGCCGTA20.25No Hit
ACTTTCGTTCTTGATCAATGAAAACATCCTTGGCAAATGCTTCCGCAGTA20.25No Hit
ACTTTCGTTCTTGATCAATGGGAACATCCCTGGCAATTGCATTCGCAGTA20.25No Hit
ACTTTCGTTCTTGATCAAAGAAAACATCCTTGGCAAATGCTTTCGCAGTA20.25No Hit
ACTTTCGTTCTTGATCAATGAAAGCATCCTTGGCAAATGCTGTCGCAGTC20.25No Hit
ACTTTCGTTCTTGATCAATGAAAGCATCCTTGGCAAATGCCTTCCCAGTC20.25No Hit
ACTTTCGTTCTTGATCAATGAGAACATCTTTGGCAGATGCTTTCGCAGTG20.25No Hit
ACTTTCGTTCTTGATTAATGAAAACATCCTTGGTAAATGCTTTCGCATAA20.25No Hit
ACTTTCGTTCTTGATTAATGAAAACATTCTTGGAAAATGCTTTCGCATTA10.125No Hit
ACTTTCGCTCTTGATCAATGAAAACATCCTTGGCAAATGCTTTCGCAGTG10.125No Hit
ACTTTCGCTCTTGATCAATGAAAACATCCTTGGCAAATGCTTTCGCAGTA10.125No Hit
ACTTTCGTTCTTGATTAATGAAAACACTCTTGGTAAATGCTTTCGCAGTA10.125No Hit
ATTTCGGTCTTGATCAATGAAAACATCCTTGGCAAATGCTTTCGCAGTAG10.125No Hit
ACTTTCGTTCTTGATCAATGAAAACGTCCTTGGCCAACGCTTTCGCAGTA10.125No Hit
ACTTTCGTTCTTGATCAATGAAAACATCCTTGACAAATGCTTTCGCAGTA10.125No Hit
ACTTTCGTTCTTGATTAATGAAAACACTCTTGGACAATGCTTTCGCAGTA10.125No Hit
ACTTTCGTTCTTGATCAATGAAAACACTCTTGGAAAATGCTTTCGCAGTA10.125No Hit
ACTTTCGTTCTTGATCAGTGAAAACATCCCTGGCAAATGCCTTCGCAGTG10.125No Hit
ACTTTCGTTCTTGATCAGTGAAAACATCCCTGGCAAATGCTTTCGCAGTT10.125No Hit
ACTTTCGTTCTTGATCAAAAAAAAAACATCCCGGGCAGATGCCTTCGCCA10.125No Hit
ACTTTCGTTCTTGATCAATGAAAACGTTCTCGGGAAACGCCTTCGCATCC10.125No Hit
ACTTTCGTTCTTGATCCTTGAAAACATCCTCGACAAATGCTTTCGCCTTA10.125No Hit
ACTTTCGTTCTTGATCAATGAAAACATCCTTGGAAAATGCTTTCGCAGTA10.125No Hit
ACTTTCGTTCTTGATCAATGAGAACATCCTTGGTAAATGCCTTCGCAATT10.125No Hit
ACTTTCGTTCTTGATCAATAAAAACATCCTTGGCAGATGCTTTCGCAGTA10.125No Hit
ACTTTCGTTCTTGATCAATGAAAACATCCTTGGCAAATGCTTTCGCATTA10.125No Hit
ACTTTCGTTCTTGATCAACGGGAACGTCCTAGTCAAATGCTTTCGCAGTT10.125No Hit
ACTTTCGTTCTTGATCAATGAAAACATTCTTGGCAAATGCCTTCACAGTA10.125No Hit
ACTTTCGTTCTTGATCAATGAAAACATCCTTGGCAGGTGCCTTCGCAGTT10.125No Hit
ACTTCGTTCTTGATTAATGAAAACATCCTGGGCAAATGCTTTCGCAGTAG10.125No Hit
ACTTTCGTTCTTGATCAATGAAAACATCATTGGCAAATGCTTTCGCAGTA10.125No Hit
ACTTTCGTTCTTGATCGATGAAAACATCATTGGGGCTGGAATTACCGCAGCTGCTGG10.125No Hit
ACTTTCGTTCTTGATCAATGAAAACATCCTTGGCAAATGCTTTCGCAGAA10.125No Hit
ACTTTCGTTCTTGATCAATGAAAACATCCGTGGCAAATGCTTTCGCAGTA10.125No Hit
ATTTCGTTCTTGATTAATGAAAACACTCTTGGAAAATGCTTTCGCAGTAG10.125No Hit
ACTTTCGCTCTTGATCAATGAAAACGTCCTTGGCCAACGCTTTCGCAGTA10.125No Hit
ACTTTCGTTCTTGATCAATGAAAACATTCTTGGCAAATGCTTTCGCAGTA10.125No Hit
ACTTTCGTTCTCGATCAATGAAAACATCCTTGGCAAATGCTTTCGCAGTA10.125No Hit
ACTTTCGTTCTTGATCAATGAAAACATAGTTGGCAAATGCTTTCGCAGTA10.125No Hit
ACTTCGTTCTTGATCAATGAAAACATCCTTGGCAAATGCTTTCGCAGTAG10.125No Hit
ACTTTCGTTCTTGATCAATGAAAACATTCTTGGCAAATGCTTTCACAGTA10.125No Hit
ACTTTCGTTCTTGATCAATAAAAACATCCTGGGCAGATGCTTTCGCAGTA10.125No Hit
ACTTTCGTTCTTGATCAATAAAAACACACTTGGCAAATGCTTTCGCAGTA10.125No Hit
ACTTTCGTTCTTGATCAAAGAAAACATCCTTGGCAAATGCTTTCGCAGTT10.125No Hit
ACTTTCGTTCTTGATTAATAAAAACATCCTGGGCAGATGCTTTCGCACTA10.125No Hit
ACTTTCGTTCTTGATCAAAAAAAACATCCTTGGCAAATGCTTTCGCAGTT10.125No Hit
ACTTTCGTTCTTGATCAAAAAAAAAACATCCCGGGGAGATGCCCTCGCCA10.125No Hit
ACTTTCGTTCTTGGTTAATGAAAACACTCTTGGAAAATGCTTTCGCAGTA10.125No Hit
ACTTTCGTTCTTGATTAATGAGAACATCCTGGGTAAATGCCTTCGCAATT10.125No Hit
ACTTTCGTTCTTGATCAATGAAAACATCCTTGAGAAATGCTTTCGCAGTA10.125No Hit
CCTTTCGTTCTTGATTAATGAAAACACTCTTGGAAAATGCTTTCGAAGTA10.125No Hit
ACTTTCGTTCTTGATTAATGAAAACATCCTGGGCAGATGCTTTCGCAGTA10.125No Hit
ACTTTCGTTCTTGATTAAGGAAAACATCCTGGGCAAATGCTTTCGCAGTA10.125No Hit
ACTTTCGTTCTTGATTAATGAAAACATCCTTGGCGAATGCTTTCGCAGTA10.125No Hit
ACTTTCGTTCTTGATCGATGAAAACATCCTTGGCAAATGCTTTCGCAGTA10.125No Hit
ACTTTCGTTCTTGATCAATGAAAACGTTCTTGGGAAACGCCTTCGCATCA10.125No Hit
ACTTTCGTTCTTGATCAATGAAAACATCCTGGGTAAATGCCTTCGCAGTA10.125No Hit
ACTTTCGTTCTTGATCGATGAAAACATGATTGGCAAATGCTTTCGCAGCA10.125No Hit
ACTTTCGTTCTTGATCAATGAAAACATCCTTGGCACATGCTTTCGCAGTA10.125No Hit
ACTTTCGTTCTTGATCAATGAAAACATCCTTGCCAAATGCTTTCGCAGTA10.125No Hit
ACTTTCGTTCTTGATCAATGAAAACACCCTTGGCAAATGCTTTCGCAGTA10.125No Hit
ACTTTCGTTCTTGATTAATGAAAACATCCTGGGCCAATGCTTTCGCAGTA10.125No Hit
ACTTTCGTTCTTGATCAATGAAAACATCCTTGGTAAATGCCTTCGCATTA10.125No Hit
ACTTTCGTTCTTGATCAATAAAAACATCCTTGGCAAATGCTTTCGCAGTT10.125No Hit
ACTTTCGTTCTTGATTAATGAAAACATCCTTGGTAAATACTTTCGCAGTA10.125No Hit
ACTTTCGTTCTTGATTAATGAAAACACTCTTGGAAAATACTTTCGCAGTA10.125No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)